indianbanksassociation Chapter 362 Song He Zen
Chapter 362 Song He Zen
Song He walked around the laboratory and chose his job, patch clamp!
The principle of patch clamp is very simple. It uses an extremely thin glass needle to pinch the cell membrane and test many properties of nerve cells.
Ion channel current, single cell current, whole cell current, membrane potential, membrane current, postsynaptic potential, etc. Tiger Balm is generally omnipotent. By measuring these messy readings, you can infer many things, such as the exact location of drug molecules. Did it work?
The principle is simple, but the actual operation process is extremely difficult!
A single cell itself is very small, not to mention grasping the dendritic plexus and axon fibers. It is as difficult as picking out the ear with a rolling pin. A single tremor is enough to tear the cell into pieces.
Moreover, the operation required fresh brain slices. Considering that he had no experience, Song He decisively recruited Fan Tao as his assistant and asked her to be responsible for killing the monkeys.
"It's a piece of cake!" Fan Tao said in a haughty tone.
As soon as an expert takes action, he will know if there is one!
Fan Tao moves very quickly, showing off the style of a professional butcher!
First find a half-dead monkey and kill it quickly while the monkey is still alive.
After taking out the brain, put it into a vibrating microtome, quickly cut out the brain slices, and soak them in artificial cerebrospinal fluid for short storage. At the same time, she had to continuously ventilate oxygen during the entire operation to ensure that the brain slices remained fresh.
Once the brain slices were extracted, Fan Tao's work came to an end.
Song He threw her a small red flower and sent her away, leaving her to operate the instrument alone.
Although it was his first time to operate it, Song He had watched the operation videos and related papers and had practiced it countless times in his mind, so he showed off his veteran style as soon as he got started!
First pull the electrode, the microelectrode of the borosilicate capillary glass tube, pull out the tip, and quickly apply glue and polish it.
Turn on the water bath mode of the instrument and add positive pressure, insert the electrode into the external liquid, quickly remove the bubbles in the liquid, and then use the amplifier software to flatten the resistor curve to zero...
Song He stared closely at the microscope, letting the glass needle penetrate the cells, confirming the strong wind connection, and applying negative pressure to break the cell membrane.
How to suck negative pressure?
Suck with your mouth.
The glass needle inserted into the cells is connected to a flexible tube, and a syringe hangs behind the flexible tube.
Song He opened his mouth to hold the end of the slender syringe, deflated his cheeks, and carefully sucked it in!
Look at the microscope...the cells are broken.
All in vain.
Failure is the mother of success, come back!
After another series of tedious procedures, Song He held the syringe in his mouth again and sucked it lightly!
The cells are broken again.
Failure is success, grandma, try again!
Suck, break, suck, break...
The operation is as fierce as a tiger, and the failed generations gradually become grandma's grandma's grandma.
Song He was confused. He didn't have much lung capacity, and he really didn't exert much force. Why did he always suck the cells into pieces? Watch other people's operation videos, it's obviously a very simple step!
He took another monkey brain specimen and completed a bunch of tedious steps.
In the last step, he stared at the syringe, hesitant to take his mouth, and his face was tangled.
"Boss, what's wrong with you?" Fan Tao passed by.
"Suction of negative pressure has failed eight times." Song He looked bitter and gestured with a pistol, "Eight times! I am speechless!"
"What's so difficult about this?" Fan Tao ran over, took a look at the microscope, and without hesitation opened his mouth to hold the syringe, his soft and white cheeks trembling slightly.
Song He stretched his head to look at the microscope and clapped his hands with joy, "Awesome! Success!"
He took it out of his pocket and threw a small red flower to Fan Tao. She wore it on her chest and left happily.
Song He reached out to take off the syringe, and his hand was wet to the touch... The syringe has not been changed or wiped for a long time.
"Fuck..." Song He realized later, with a stunned look on his face, and turned to look at Fan Tao in the distance. She was sitting in front of the computer and processing data intently.
Forget it, it’s better not to tell her, sometimes people are happier when they know less.
Song He kept busy recording data and officially started work.
From time to time, he called Fan Tao over and asked her to help suck the negative pressure with her mouth.
For the next three days, he focused on the patch clamp. The dying monkeys were not enough, so he got some mice to test the drug, and measured the brain slices of the mice after taking the drug to supplement the data.
Every morning he took Zuo Gaoming and Lou Jinghui to collect monkeys, fifteen of them every day, and most of them could be fed. Soon there were nearly a hundred monkeys in the laboratory, and the transparent incubation box completely occupied all the space in the laboratory. Achieved the spectacular situation of "more monkeys than people"!
There were more monkeys. Although the researchers were tired and dizzy from taking care of the monkeys, the progress of the drug trial was indeed several times faster!
There is a piece of white paper pasted on the easternmost wall, occupying most of the wall.
Every time the team members complete a drug test, they draw a small circle on the paper with a marker and record a summary of the data.
Each small circle extends out branches, leading to more small circles with similar relationships. Some branches only grow a few inches and then stop because the efficacy of the medicine is really poor. Some branches keep growing upward in a curved way. Continuously revise and improve the efficacy of medicine.
From a distance, the data tree formed by small circles looks like a wildly growing ivy.
Time flew by, and five days passed before I knew it.
The wallpaper changed drastically. Ivy covered half of the wallpaper area, and thousands of sets of data were arranged on it, which was dazzling.
After dinner, Song He stood in front of the wall, looking at the data on the wall and meditating.
From time to time, he took out a mental arithmetic capsule or comprehension capsule and stuffed it into his mouth to speed up his brain.
The brain cells are running wildly, and the data on the entire wall can be seen at a glance. Complex molecular structures are rolling past in front of the eyes, and a large number of papers are recalled from the head!
If there is a library-like memory area in the human brain, then strong winds are blowing in Song He's mind at this moment. Thousands of pages are blown away by the wind, circling all over the sky like a flock of seagulls, and each pair of bird wings has a drug molecule printed on it. thesis!
Song He stood there from 7pm to 11pm.
The busy team members in the laboratory were whispering to each other and discussing the boss who was behaving strangely.
"What are you doing, boss? Thinking about your mistakes while facing the wall?"
"It's been several hours. Even if I kill someone, I have to finish thinking about it! I think the boss's demeanor is a bit like Bodhidharma practicing meditation. He wants to escape into Buddhism."
"I'm looking at the data, I guess, but why do you have to look at it for so long? Can you see the flowers?"
"I hope the boss will look at it and suddenly clap his hands and say that he has found a new drug. Let's get off work!"
"How is it possible? Although the boss is strong, he is not that powerful. If the new drug is really found, we are the ones doing the experiment to find it first."
Suddenly, Song He clapped his hands violently!
"Gather for a meeting! I've probably found the route!" he shouted.
Everyone in the laboratory was shocked and hurriedly gathered around Song He, waiting eagerly.
"Judging from the data, we are very close to the final medicine!" Song He looked at the circle on the wall, "I said medicine, you go and test it, the faster the better! Except for the medicine I asked for, no more It’s a waste of time making other medicines!”
"Understood!" Everyone was hesitant and a little excited and nervous.
"Lou Jinghui! Your team is going to make drugs C101, C108, and C236 now!"
"Teacher Lu, your team is going to make medicines E780, E788, and E795!"
"Ji Tianhe, your team's mission is drugs G340, G351, G362, and G377!"
Song He waved his hand suddenly and everyone dispersed quickly.
A burst of monkey howling sounded in the laboratory.